目的 观察西瓜藤不同部位提取物对糖尿病小鼠的降血糖作用。方法 采用小鼠腹腔注射四氧嘧啶法建立糖尿病小鼠模型，采用西瓜藤不同提取物喂养干预治疗，观察其对小鼠空腹血糖、葡萄糖耐量;血清中胰岛素水平、三酰甘油(TG)、总胆固醇(TC)、一氧化氮(NO)和诱导型一氧化氮合酶(iNOS)含量和肝组织匀浆中一氧化氮和一氧化氮合酶水平的影响;并观察小鼠胰腺组织病理学改变。结果 西瓜藤乙酸乙酯高剂量组和石油醚高剂量组总胆固醇显著性降低，三酰甘油也有所降低，血糖水平及糖耐量均有所改善，胰岛素含量显著升高，一氧化氮和一氧化氮合酶含量显著降低。结论 西瓜藤乙酸乙酯部位、石油醚部位提取物对四氧嘧啶致糖尿病小鼠具有一定的降血糖作用，其作用机制可能与促进胰岛素的释放及降低一氧化氮和一氧化氮合酶含量有关。
Abstract
OBJECTIVE To investigate the hypoglycemic effects of different fractions from Citrullus lanatus vine on diabetic model induced by alloxan in mice. METHODS Mouse were used to establish diabetic models by peritoneal injection administration of alloxan. The different fractions from Citrullus lanatus vine was applied as raw materials for potential marine drug. The levels of fasting blood glucose (FBG), fasting blood insulin (FBI) and glucose tolerance were detected by automatic blood glucose device. The concentrations of total cholesterol (TC), total glycerin (TG), nitric oxide (NO) and nitric oxide synthase (iNOS) in blood were respectively measured. The concentrations of nitric oxide (NO) and nitric oxide synthase (iNOS) in liver homogenate were determined. Pathology of pancreas was observed. RESULTS The ethyl acetate high dose group and petroleum ether high dose group all significantly decreased the levels of TC, TG, NO and iNOS. Meanwhile, blood glucose level and glucose tolerance were improved. Insulin level was also improved. CONCLUSION Ethyl acetate group and petroleum ether group of Citrullus lanatus vine shows significant hypoglycemic effect on diabetic model induced by alloxan in mice. The possible mechanism is to promote the insulin release and decrease the levels of NO and iNOS.
关键词
西瓜藤 /
糖尿病 /
降血糖作用
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Key words
Citrullus lanatus vine /
diabetic /
hypoglycemic effect
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中图分类号：
R965
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参考文献
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脚注
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基金
广西壮族自治区科技厅科技基础条件平台建设项目(10-046-04)；广西科技厅科技攻关项目(桂科攻10124008-12)
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